Hello, and i hope I can help you today. Pregnancy symptoms vary widely between women, and many of the same symptoms people associate with pregnancy like irregular bleeding, breast tenderness, bloating, nausea and mood changes can also been used by other hormonal irregularities, such as missed ovulation. In addition, women can have abnormal periods from the stress of worrying about pregnancy. Therefore, there is no way to know if you are pregnant based on symptoms. So I would consider repeating your pregnancy test just in case, but if you performed it correctly with your first morning urine, the results should be accurate. So you may just need to wait a little bit and your breast tenderness should resolve when eventually your period comes. If you miss your period for more than 3 months and are not pregnant, you could consider seeing your medical doctor for a hormonal evaluation. I hope I was able to adequately answer your question today and that my advice was helpful. Best wishes, Dr. Brown