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Is A P 24 HIV 1 And 2 Test Conclusive At 70 Days Post Exposure?

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Posted on Mon, 18 May 2015
Question: male 32 1 min unprotected penetration but no ejacualtion in vagina not with commercial sex worker but with maid servant . after that had unprotected sex with gf but did not exaculate post 18 days i did immuno cromotography test ( negative) post 21 days hiv 1 and hiv 2 p24 ( negative ) post 24 days hiv 1 and hiv 2 p24 ( negative) post 24 days pcr viral load came after two days saying target no detected . post 33 days days hiv 1 and hiv 2 p24 negative post 40 days western blot negative for both hiv 1 and hiv 2 but ( p51,p55, p 66 detected this is confusing ) .post 42 days hiv 1 and hiv 2 p24 negative .girl friend test after 30 days hiv and hiv 2 p24 negative .i have autoimmune hepatits ( take prednisolone 5 mg and immuron 50 mg since 1994 ) and HBV ( blood transfusion for which i am on tenvir 300 mg for 4 years) did LFT test post exosure results were good !!
on 68 or 69 day did western blot which came out negative ( this time no bands detected all negative ) , 70 days day did p24 hiv1 and hiv 2 negative !! today 74 day i did pcr rna waiting for result !! plez help
anxiety is killing me ..
doctor
Answered by Dr. Sankaranantham Murugan (1 hour later)
Brief Answer:
Tenvir, potent ART abort if there was any infection. Nothing to worry

Detailed Answer:
Hi,
Welcome to HCM.
Thanks for posting your query.
First of all why you had numerous tests for HIV?
Your servant maid would not have HIV infection at all. Then where is the question of HIV for you? Your girl friend also tested negative for HIV combo test.

HIV 1 and 2 / P 24 Antigen test negative after 24, 33,42 and 70 days after high risk contact along with undetectable viral load with RNA PCR test on 24 days clearly ruled out HIV 1 infection (which is prevalent more than 99.5% everywhere in the world except in certain parts of Africa). Antibody part of the Combo test and western blot and immuno-Chromatography test become more conclusive after 12 weeks.
In your case as you are on steroids and Tenvir it may get delayed still more.
At the same time Tenvir 300 mg tablets taken by you, have effect to abort HIV 1 and 2 infection, if any, infection have occurred to you during that exposure as it has anti-retroviral effect both on HIV 1 and 2.
So there is no chance for you get HIV infection.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (26 minutes later)
good day doctor thx for your reply ... really assuraing .. i know i did a stupid mistake by having unprotected sex twice!!

1)my question do i need to get my girlfriend tested again ? 2)cause if i had it she surely would have right ? 3) do i need to get tested again !


thx a lot for your advice !! i have lost sleep and 3 months now stopped working out and living .. i live in fear daily !!
doctor
Answered by Dr. Sankaranantham Murugan (5 hours later)
Brief Answer:
GF unlikely to get infected. Rule out HIV 2 infection after 3 months

Detailed Answer:
Hi,
Welcome back.

1.As you are on Tenvir, if there was any infection, it would have been aborted as I had mentioned already. So there is no chance for your girl friend to acquired HIV infection from you.
2. All exposures are not necessarily infectious.There are 30-40% of the spouses of HIV positive people remain non-reactive in spite of their regular and prolonged sex contacts with their spouses and they are called as Sero- discordant couple. Likewise even if you have infection there are chances for her to remain uninfected in spite of your sex relationship.
3. Having undergone so many tests so far, it is better to rule out HIV 2 infection (less than 0.5% chance) by a rapid or ELISA test after 3 months and after 6 months as you are on steroid.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (18 minutes later)
thx doctor for your reply !! Will do after 3 months ! I will br touch and keep you updated ! God bless you sir
doctor
Answered by Dr. Sankaranantham Murugan (7 hours later)
Brief Answer:
Welcome You are free from HIV 1 infection

Detailed Answer:
Hi XXXX,

You are almost HIV free. Be confident. Nothing to worry.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (2 days later)
Good day doctor ! Before contacting u we had run p24 combo test and western blot on my gf today they bothcame negative ! This is post 66 days exposure to me , is this good news ? Waiting for my pcr test result tommrow post 74 days fingers crossed !
doctor
Answered by Dr. Sankaranantham Murugan (39 minutes later)
Brief Answer:
Your girl friend also free from HIV 1 Infection

Detailed Answer:
Hi,
Welcome back.
Definitely it is a good news. Your girl friend's tests also ruled out the HIV 1 infection in her.
Your test also will ensure that you are free from HIV infection
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Vinay Bhardwaj
doctor
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Follow up: Dr. Sankaranantham Murugan (20 hours later)
good doctor !! greeting s!! i have attached my PCR report !!

please let me know what does it say .
2) sir i want to rule out all SIT etc what test to do
3) after 90 days should i do p24 combo or some other test ?

Regards XXXXXXX
doctor
Answered by Dr. Sankaranantham Murugan (2 hours later)
Brief Answer:
VDRL, HBSAg, HCV, Urine examination,HSV and Chlamydial test to rule out STI

Detailed Answer:
Hi,
Welcome back.

1.Your RNA PCR result reflects that it can not detect any HIV virus in your blood to its maximum possible extent.

2.Urine examination, Blood test for Syphilis, Hepatitis B and C, HSV 1 and 2 Ig G and M test are enough to rule out STI.
3. HIV 2 is yet to be ruled out. Combo test or any other antibody detecting test by 12 weeks would rule out HIV 2 also which may be a rarest and remote possibility.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (10 minutes later)
Hcv was ruled out it came negative sir ! Rest i will i get it done asap sir ! But this pcr is good news rite sir?
doctor
Answered by Dr. Sankaranantham Murugan (7 minutes later)
Brief Answer:
You are free from HIV 1 INFECTION.

Detailed Answer:
Hi,
Welcome back.
HIV RNA PCR undetectable viral load after 74 days is definitely a good result.
Keep yourself relaxed.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (13 hours later)
Hi sir have given blood for all except chlamydial cause they wanted to knwo exactly cause even in chl..there are different types so wanted to ask u before i went for it !
doctor
Answered by Dr. Sankaranantham Murugan (2 hours later)
Brief Answer:
screening for STD s is recommended

Detailed Answer:
Hi
Welcome back
Screening for all STDS is good. All tests are expected to come as negative. Good day to you
Dr S Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (15 minutes later)
No sir what i meant was they were saying which type of chlamydial test or i guess detection method or something ..cause their list show apart from basic chy. Detection there are differebf judt like for hiv pcr etc types ..so i thought i should ask you before
doctor
Answered by Dr. Sankaranantham Murugan (1 hour later)
Brief Answer:
Chlamydial PCR is preferable

Detailed Answer:
HI,
Welcome once again.

There are different serovars of Chlamydia causing a wide range of diseases like Trachoma (A,B,C), Lymphogranuloma (L1,L2,L3), inclusion conjuctivitis and Non gonococcal urethrits (D-K).
But usually we don't prefer to find the serovars separately. Only we prefer to fond whether Chlamydial infection is there or not.
PCR test for Chlamydia is preferable than with others.
Dr S.Murugan

Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (2 hours later)
Sir just got VDRL report says non reactive! Rest sill get tommrow or saturday !what
doctor
Answered by Dr. Sankaranantham Murugan (3 hours later)
Brief Answer:
Negative VDRL ensures that you are free from Syphilis

Detailed Answer:
Hi,
Welcome.
You are free from Syphilis.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (23 hours later)
Hi sir! Just wanted to wish u good day ! No question today :-)! Your support has been great and helpful , god bless you sir ! My other reports will come tommrow or day after ! Will be updating u as usual !
doctor
Answered by Dr. Sankaranantham Murugan (8 minutes later)
Brief Answer:
Safe sex is always good.

Detailed Answer:
Hi XXXX,
you will get all negative results for the other test also. But here afterwards try to stick to safe sex to avoid unnecessary mental tension. Expecting to share your your good news in the coming days.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (19 hours later)
Dear sir

have attached my three reports ! kindly advice thx
doctor
Answered by Dr. Sankaranantham Murugan (4 hours later)
Brief Answer:
HIV 1, SYPHILIS, HSV and HCV ruled out beyond any doubt.

Detailed Answer:
Hi,
Welcome back.
All results were in favor. You are free from Syphilis, HSV 1 and 2 infections, HIV 1 infection and HCV (as you had mentioned already). You had ruled out almost all major blood born diseases and STDs. These test reports after 74 days are conclusive.
Relax and be happy. Forget the past incidence. HIV 2 also will be likely to be non reactive.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Prasad
doctor
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Follow up: Dr. Sankaranantham Murugan (16 minutes later)
Yes sir will be wiser now !!! Sir when do i get hiv 2 test ? After 12 weeks (which i will be completing on monday ) or when sir !
doctor
Answered by Dr. Sankaranantham Murugan (8 minutes later)
Brief Answer:
HIV 2 can be ruled out by antibody test 90 days after the contact.

Detailed Answer:
Hi,
Welcome once again.
I will say 12 weeks is final to have the test. But better to have the test by 90 days after the contact, so that we need not have any doubt about the test report.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Prasad
doctor
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Follow up: Dr. Sankaranantham Murugan (44 minutes later)
Ok sir will do for sure :-) ! Good night sir !
doctor
Answered by Dr. Sankaranantham Murugan (6 hours later)
Brief Answer:
A good day ahead to you

Detailed Answer:
Hi,
Welcome and a good day to you!

Above answer was peer-reviewed by : Dr. Raju A.T
doctor
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Follow up: Dr. Sankaranantham Murugan (35 hours later)
Hi sir ! Hope u doing good ! Will be get tested on thursday or friday since will be in XXXXXXX ! Keeping my fingers crossed ! Should i go for western blot ,pcr or p24 combo sir ?
doctor
Answered by Dr. Sankaranantham Murugan (46 minutes later)
Brief Answer:
ELISA. Rapid or 4th generation COMBO test preferred.

Detailed Answer:
Hi,
Welcome back.
HIV PCR test and P 24 Antigen test would not rule out HIV 2 infection.
4th generation COMBO test Ab 1/2 P 24 antigen test or any Rapid test, ELISA test can be done All are almost equally good and reliable. Western blot also can be done But it is less sensitive for HIV 2 is concern.
DR S.Murugan

Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (2 days later)
hi sir just wanted to greet you , hope u are doing good !

i will be going for the test tommrow or dayafter ! also all the test i have done are form dr lal path lab and SRL , hope these are good labs and all test done were 4th gen combo or dual .so i will go for the same test sir from these labs ?

just a little nervous though sie and just praying !!
doctor
Answered by Dr. Sankaranantham Murugan (19 minutes later)
Brief Answer:
LAL PATH and SRL labs are highly reliable laboratories.

Detailed Answer:
Hi,
WELCOME once again.
Lal PATH LAB and SRL LABS are the best and reliable laboratories in XXXXXXX You can go with them confidently.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (27 hours later)
Dear sir

87 -88 days p 24 combo test hiv1 and hiv 2 anti body screening test (4th gen )is non reatice at lal path lab XXXXXXX ...have asked them to do weaternblot and pcr rna , report awaited for these two monday ! Plz advice further cource of action !
doctor
Answered by Dr. Sankaranantham Murugan (3 hours later)
Brief Answer:
No further test is necessary. You are free from both HIV 1 and 2 infection.

Detailed Answer:
Hi,
Welcome back.
Negative HIV 4th generation test after 12 weeks (84 days) clearly ruled out both HIV 1 and 2. No more test is necessary for you. You do not need any further course of action and just forget about the incident and relax. Try to stick on safe sex in your future to avoid unnecessary mental tension.
Have good days ahead.
Dr S..Murugan
Above answer was peer-reviewed by : Dr. Vinay Bhardwaj
doctor
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Follow up: Dr. Sankaranantham Murugan (29 minutes later)
Dear sir thx a lot ! Sir will take your advice its a wake up call ! Sir i cant thank you enough for this really ! God is great ! Sir where can i look u up apart from this forum or virutal environment and personely thank you ! I would really like to stay kn touch with u sir !
doctor
Answered by Dr. Sankaranantham Murugan (6 hours later)
Brief Answer:
You are most welcome

Detailed Answer:
Hi XXXX,

I am happy to see you back with your normalcy. You are welcome. If you have no more questions to ask, you can close this conversation. You can meet me at SHIFA Hospitals, XXXXXXX Tamilnadu on all weekdays between 5-7.30 pm.
Dr S.Murugan
Above answer was peer-reviewed by : Dr. Yogesh D
doctor
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Follow up: Dr. Sankaranantham Murugan (2 days later)
Hi sir was travelling and also waiting for pcr and weaten blot report ..pcr say no target detected and weatern blot all negative sir !

I will see you form sure at shifa !
Sir my questions ..
1) any specific condoms which offer good protection
2) can i resume normal life ( workout , running )
3) does my gf need testing ?

Thx sir !
doctor
Answered by Dr. Sankaranantham Murugan (29 hours later)
Brief Answer:
you are free from HIV 1and2. Resume normal activities

Detailed Answer:
Hi,
Welcome back
RNA PCR undetectable and Combo tests non-reactive after 12 weeks confirm that youare free from both HIV 1and 2.
You can resume your normal sexual activities.
Your girlfriend not necessary to be tested
Dr S Murugan
Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
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Follow up: Dr. Sankaranantham Murugan (26 hours later)
Dear sir , as i said i really cant thank you enough ! God bless u sir ! Rest assured will be wise and not stupid ! Will be in touch sir ! See ya soon
doctor
Answered by Dr. Sankaranantham Murugan (3 hours later)
Brief Answer:
Most welcome

Detailed Answer:
Hi,
You are welcome at any time.
Wish you have very good days ahead.
Dr S.Murugan
Note: For further follow up on related General & Family Physician Click here.

Above answer was peer-reviewed by : Dr. Chakravarthy Mazumdar
doctor
Answered by
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Dr. Sankaranantham Murugan

HIV AIDS Specialist

Practicing since :1974

Answered : 3110 Questions

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Is A P 24 HIV 1 And 2 Test Conclusive At 70 Days Post Exposure?

Brief Answer: Tenvir, potent ART abort if there was any infection. Nothing to worry Detailed Answer: Hi, Welcome to HCM. Thanks for posting your query. First of all why you had numerous tests for HIV? Your servant maid would not have HIV infection at all. Then where is the question of HIV for you? Your girl friend also tested negative for HIV combo test. HIV 1 and 2 / P 24 Antigen test negative after 24, 33,42 and 70 days after high risk contact along with undetectable viral load with RNA PCR test on 24 days clearly ruled out HIV 1 infection (which is prevalent more than 99.5% everywhere in the world except in certain parts of Africa). Antibody part of the Combo test and western blot and immuno-Chromatography test become more conclusive after 12 weeks. In your case as you are on steroids and Tenvir it may get delayed still more. At the same time Tenvir 300 mg tablets taken by you, have effect to abort HIV 1 and 2 infection, if any, infection have occurred to you during that exposure as it has anti-retroviral effect both on HIV 1 and 2. So there is no chance for you get HIV infection. Dr S.Murugan