Immunophenotyping

What is Immunophenotyping?

Immunophenotyping is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue), cell suspension, etc. An example is the detection of tumor marker, such as in the diagnosis of leukemia. It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane. By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined. The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per second. The whole procedure can be performed on cells from the blood, bone marrow or spinal fluid in a matter of a few hours.

Questions and answers on "Immunophenotyping"

My father had vertigo in Nov'16. Post which we got the complete health check up done. In health check up we came to know that his heart is pumping...

doctor1 MD

Brief Answer:
Immunophenotyping should be done .

Detailed Answer:
Hi, dear
I have gone through your question.
I can understand your concern.

You...

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hi What is leukemil lymphoma

doctor1 MD

Brief Answer:
It is mature t cell neoplasm.

Detailed Answer:
Hi, dear
I have gone through your question. I can understand your concern.
Leukemia...

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My husband has CLL but his bone marrow is working properly and they say it is not the CLL.
He has a high white blood cell count and low red blood...

doctor1 MD

Brief Answer:
Reports needed. May need immunophenotyping.

Detailed Answer:
Hi, dear

I have gone through your question. I can understand your...

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