Immunophenotyping

What is Immunophenotyping?

Immunophenotyping is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue), cell suspension, etc. An example is the detection of tumor marker, such as in the diagnosis of leukemia. It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane. By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined. The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per second. The whole procedure can be performed on cells from the blood, bone marrow or spinal fluid in a matter of a few hours.

Questions and answers on "Immunophenotyping"

My father had vertigo in Nov'16. Post which we got the complete health check up done. In health check up we came to know that his heart is pumping...

doctor1 MD

Brief Answer:
Immunophenotyping should be done .

Detailed Answer:
Hi, dear
I have gone through your question.
I can understand your concern.

You...

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hi What is leukemil lymphoma

doctor1 MD

Brief Answer:
It is mature t cell neoplasm.

Detailed Answer:
Hi, dear
I have gone through your question. I can understand your concern.
Leukemia...

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My husband has CLL but his bone marrow is working properly and they say it is not the CLL.
He has a high white blood cell count and low red blood...

doctor1 MD

Brief Answer:
Reports needed. May need immunophenotyping.

Detailed Answer:
Hi, dear

I have gone through your question. I can understand your...

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Recent questions on  Immunophenotyping

doctor1 MD

Hi there, Wondering if you could interpret some flow cytometry results for me. I am a 27-year-old female who recently underwent lingual...

doctor1 MD

My father had vertigo in Nov 16. Post which we got the complete health check up done. In health check up we came to know that his heart is pumping...

doctor1 MD

Hello dear doctor, i would like to know what does this report mean? I took it today and last 10 days I was having fever caused by infection. During...

doctor1 MD

My mother is suffering fro AML but subtype is not clear . All kind of diagonosis has been done including immunophenotyping. Every one is telling...

doctor1 MD

my nieces is 24 years old, she was down with flu & fever and after few days back with CBC test, she diagnostic with AML Type M1 and M2,her bone...

doctor1 MD

Hello,
My 21 year old brother had a lump in his gum for about 2 years. Two weeks ago, when we visited the dentist, he said that the lump must be...

doctor1 MD

My husband is 30 years old, 5'6", 185lbs. For the past 4 months he has had an elevated white blood cell count. He has been on several different...

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