Immunohistochemistry

What is Immunohistochemistry?

Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC takes its name from the roots "immuno," in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). The procedure was conceptualized and first implemented by Dr. Albert Coons in 1941.

Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis). IHC is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.

Visualising an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction (see immunoperoxidase staining). Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein or rhodamine (see immunofluorescence).

Questions and answers on "Immunohistochemistry"

My mom had bloody sputum past couple of months and we have done many tests to diagnose problem including biopsy, it was identified that, oozing...

doctor1 MD

Brief Answer:
Yes, it points to words cancers.

Detailed Answer:
Hello,

Description few cells mentioned in report is pointing towards cancers.But...

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yes. I just had surgery for met melanoma all results were neg accept lym notes they toke 3 out amd they were post. 1st one was 2mm then went to 1mm...

doctor1 MD

Brief Answer:
You need further immunotherapy!

Detailed Answer:
Hi,

With positive lymph nodes, you need to receive systemic immunotherapy for better...

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What kind of blood tests would you order for CLL other than a cbc ? I have had this for 15 years. My white blood count is about 140.

doctor1 MD

Brief Answer:
Hello dear. Flowcytometry is indicated

Detailed Answer:
Hello dear. Welcome to HCM. So in order to diagnose cll, one needs a...

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Recent questions on  Immunohistochemistry

doctor1 MD

What kind of blood tests would you order for CLL other than a cbc ? I have had this for 15 years. My white blood count is about 140.

doctor1 MD

I have been on this site for several years and my worse fear came to pass. Since May of this year I was diagnosed with locally advanced pancreatic...

doctor1 MD

I was diagnosed with tumors on my omentum the largest of which is 3cm what could be the possible causes. I have crohns disease and all but 6 inches...

doctor1 MD

Hi...latest reports of biopsy states that "Section shows hyalinised fibrocollagenous fragments infiltrated by liner trabeculae, nests and ill...

doctor1 MD

hi I am worried ecause i went to my dermatologist because i have alot of light pink almost skin colored spots growing around my belly,back,chest...

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Hi Doctor, My father aged 63 yrs has a tumour @ right supravaculear (Size 3 Cm) in ight side of the neck. After FNAC it was confirmed as Squamacell...

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