Electrophoresis

What is Electrophoresis?

Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. This electrokinetic phenomenon was observed for the first time in 1807 by Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate. It is ultimately caused by the presence of a charged interface between the particle surface and the surrounding fluid. It is the basis for a number of analytical techniques used in biochemistry for separating molecules by size, charge, or binding affinity.

Electrophoresis of positively charged particles (cations) is called cataphoresis, while electrophoresis of negatively charged particles (anions) is called anaphoresis. Electrophoresis is a technique used in laboratories in order to separate macromolecules based on size. The technique applies a negative charge so proteins move towards a positive charge. This is used for both DNA and RNA analysis. Polyacrylamide gel electrophoresis (PAGE) has a clearer resolution than agarose and is more suitable for quantitative analysis. In this technique DNA foot-printing can identify how proteins bind to DNA. It can be used to separate proteins by size, density and purity. It can also be used for plasmid analysis, which develops our understanding of bacteria becoming resistant to antibiotics.

Questions and answers on "Electrophoresis"

9 yr boy - with no symptoms and signs wbc 6.8 k/ul Rbc 5.9 M/uL HGB 13 g/dl HCT 37.7 % MCV 63.7 fl MCH 22.4 pg MCHC 35.2 g/dL RDW 18.1 % PLT 362...

doctor1 MD

Hi,
Thank you for your query on Healthcare Magic,
The hemoglobin, WBC count, RBC count, Platelet count, RBC morphology and concentration and the...

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good afternoon doctor

my mother suffering with kidney problem means her creatine level - 3.5 now
one of the nephrologist suggest her to do usg kub...

doctor1 MD

Hi, Thanks for using HCM.
USG KUB means ultrasonography test of kidney, ureter and bladder, where the structure of these organs can be visualised...

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CBC shows slightly low hemoglobin , low MCV but high RBC hgb 12.1 (13.2-17.1), mcv 64.4 (80-100), mch 20.1 (27-33), rbc 6.03 (4.2-5.8) Not...

doctor1 MD

Hello,

Your report is showing slightly low hemoglobin low MCV. low MCH . It is called Microcytic Hypochromic Anemia . It means size of RBC is small...

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Recent questions on  Electrophoresis

doctor1 MD

good afternoon doctor

my mother suffering with kidney problem means her creatine level - 3.5 now
one of the nephrologist suggest her to do usg kub...

doctor1 MD

My 6 years old son was suffering from fever for 7 days and advised for CBC test. The following is the result of the same: Hemoglobin - 10.10(Ref...

doctor1 MD

CBC shows slightly low hemoglobin , low MCV but high RBC hgb 12.1 (13.2-17.1), mcv 64.4 (80-100), mch 20.1 (27-33), rbc 6.03 (4.2-5.8) Not...

doctor1 MD

9 yr boy - with no symptoms and signs wbc 6.8 k/ul Rbc 5.9 M/uL HGB 13 g/dl HCT 37.7 % MCV 63.7 fl MCH 22.4 pg MCHC 35.2 g/dL RDW 18.1 % PLT 362...

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