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Trying to conceive. Semen analysis test shows zero sperm count. Delay in sample reaching lab. Is there any guidelines to follow while collecting semen samples?

Hi doctor, I am 26 and my husband is 31. We are married for 2 years and we are trying for concieve for the past 4 months. We are working in two different places and hence we were together only for a few days each month. Now i am on a long leave to try for conception . As a start, my husband did a semen analysis on tuesday which came out with a report showing zero sperm count. He took the sample from home, and transported it to the lab in an air conditioned vehicle and it took almost 30 mnts delay. Are there chances that due to this the sperm could have died off by the time it reached the lab?? Just to know if there is any such kind of chances and the sample collection guidelines that should be followed while collecting semen samples.? We areanyways planning to repeat the test in another lab tommorrow.
Sun, 24 Jun 2012
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Lab Tests
General & Family Physician 's  Response
Your chances of conception will increase once you start staying together regularly.however a zero sperm count is a cause for worry for which get the semen tested again at some different lab.
How to collect semen sample
If you collected your sample in a place other than your clinic, you need to get it to the laboratory within one hour after ejaculation because sperm do not have a long life outside of the body and at different temperatures. Delays in delivering semen and exposure to various temperatures will results in lower overall motile sperm count and poor semen cryopreservation.

Your semen sample shouldn’t be exposed to any extreme temperatures and, in fact, should be kept as close to body temperature as possible. The sperm motility value will be inaccurately low if the semen sample gets cold and or if it gets too hot.

Keep your specimen container upright in a plastic bag, with the lid securely tightened. The specimen should not be placed in your partner’s purse, or in your pocket or briefcase. Keep it as close to your body as possible, perhaps by tucking it under your clothing on the way to the clinic.
You will need to refrain from any sexual activity for at least two days, but not more than 10 days before you collect your sample. This means no sex or no ejaculation of any kind, including masturbation.

Longer or shorter periods of abstinence may result in a lower sperm count or decreased sperm motility. Samples produced after two days of abstinence will usually have the highest numbers of motile sperm with the greatest forward velocity, when compared to samples produced after shorter or longer abstinence.
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