Criteria for test reading in HIV
It is my pleasure and privilege to answer your query.
Your question is quite elaborate and informative one. Clarification of doubts regarding your query requires some microbiological and molecular knowledge.
1.In any person we may get numerical values of more than 1, because test which
they have done is a qualitative one.Cutoff values is calculated based on values in hundreds of serum samples from uninfected persons. Even normal person may have value more than 0 and less than cutoff value because of non specific antigen and antibody reactions.
2.Dropped levels of P 24 is not possible in present case, because test is done within documented window period of 3 months. Antigen level may fall after seroconversion
because of formation of antigen and antibody complex.
P24 antigen is a early marker of infection as it can be detected as early as 3 weeks after possible exposure.Levels may increase until serconversion.
3.If the test is negative, different levels in different persons may be because of non specific antigen antibody reaction in the individuals.
4.Non infected persons will have different levels of antigen mainly because of non specific antigen antibody reaction and the antibody used to detect the P24 antigen (Horse or Goat) in the test.We cannot correlate numerical values with antigen levels as test done is a qualitative one and not the quantitative one.
5.Still if you any doubts, repeat the test with different principle like P24 Ag ELISA
and Ab ELISA.
Antibody ELISA can be repeated after 3 months and confirm that if it comes negative nothing to worry, because it is suggestive of not having any HIV virus or antibodies
Hope I had cleared majority of doubts and i know still you have got doubts, because some of them require molecular understanding. I have tried my level best to answer your query.