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Is ELFA Test Done Post 14 Days Of Exposure Conclusive?

Posted on Sat, 25 Jun 2016

Question: sir, i had protective vaginal sex for less than a 30 sec and unprotective oral sex(licked vagina for 3 times)

i tested for elfa at 14th day of exposure it came negative(0.05).

can u pls tell me i am safe or should i test for another time?

Answered by Dr. Indu Kumar (1 hour later)

Brief Answer:
ELFA done at 14th day may be false negative.

Detailed Answer:
Hello,
Thanks for writing to HCM,
I've gone through your query and understand your concern,

You are almost safe due to act.
Chances of transmission of HIV is very less in protected vaginal intercourse.
Oral sex is also not a major way of infection transmission but it is not zero.
So, it is important to exclude HIV completely.

ELFA done at 14th day may be false negative. It may detect up to 95% of infections at 28 days and more than 99.99% of infections at 90 days.

So,I suggest for repeat test after 3 months,this is only for our extra precaution.

Get well soon.

Hope I have answered your query.
Further queries are most welcome.

Take Care
Dr. Indu XXXXXXX

Above answer was peer-reviewed by : Dr. Sonia Raina

Follow up: Dr. Indu Kumar (2 hours later)

Thanks for advice
As I am real stressed,Can I undergo HIV RNA PCR now(18th day of exposure)
1Will u pls tell where it be performed at XXXXXXX
2.how sensitive is this test.

Answered by Dr. Indu Kumar (3 hours later)

Brief Answer:
It is up to 99 % sensitive.

Detailed Answer:
Hello,
Thanks for writing to HCM,
I've gone through your query and understand your concern,

If you are so stressed then you can go for RNA PCR test.This is ideal in this period.It is up to 99 % sensitive.

It can be done in any renowned diagnostic center e.g Lal laboratory,SRL Ranbaxy etc.

Get well soon.

Hope I have answered your query.
Further queries are most welcome.

Take Care
Dr. Indu XXXXXXX

Above answer was peer-reviewed by : Dr. Yogesh D

Follow up: Dr. Indu Kumar (46 hours later)

Sir, firstly I apologise u that I did not undergo pcr rna test as the lab person told it done only for Dna not rna
So I want to undergo elfa test at 23rd day of exposure it reliable
Anyway I will undergo test after 28 days of exposure
Pls advice me as I am in terrible depression

Answered by Dr. Indu Kumar (15 hours later)

Brief Answer:
You may undergo combined antigen P24 and HIV I and II antibody test.

Detailed Answer:
Hello,
Thanks for writing to HCM,
I've gone through your query and understand your concern,
Sorry for replying late

PCR DNA test is used to detect viral load in already infected persons.It is not relevant in your case.
In window period,either PCR RNA or P24 antigen test is used.
Since you are exposed 28 days before,you may undergo combined P24 antigen and antibody test.It is highly sensitive.

You may need repeat antibody test(ELFA)after 3 months.It will be confirmatory and can detect infection in most of the cases.

Get well soon.

Hope I have answered your query.
Further queries are most welcome.

Take Care
Dr. Indu XXXXXXX

Above answer was peer-reviewed by : Dr. Arnab Banerjee

Follow up: Dr. Indu Kumar (2 hours later)

Thanks for your reply.
What about doing pcr rna or elfa test at 23 day of
Exposure.
I am really stressed

Answered by Dr. Indu Kumar (1 hour later)

Brief Answer:
You may go for fourth generation dual test.

Detailed Answer:
Hello,
Thanks for writing to HCM,
I've gone through your query and understand your concern,

PCR RNA can be done at 23 days.It is highly sensitive.
ELFA may be up to 90 % sensitive.
You may go for fourth generation dual test i,e both antigen P24 and antibody test.

Get well soon.

Hope I have answered your query.
Further queries are most welcome.

Take Care
Dr. Indu XXXXXXX

Above answer was peer-reviewed by : Dr. Sonia Raina